双击DataGridView进入事件
private void dataGridView1_CellContentClick(object sender, DataGridViewCellEventArgs e) { int count = dataGridView1.Rows.Count; for (int i = 0; i < count; i++) { DataGridViewCheckBoxCell checkCell = (DataGridViewCheckBoxCell)dataGridView1.Rows[i].Cells[0]; Boolean flag = Convert.ToBoolean(checkCell.Value); if (flag == true) { checkCell.Value = false; } else { continue; } } }