同样的名为read_1.fa 的fasta文件,里面有若干序列,如:
>@r1
TGAATGCGAACTCCGGGACGCTCAGTAATGTGACGATAGCTGAAAACTGTACGATAAACNGTACGCTGAGGGCAGAAAAAATCGTCGGGGACATTNTAAAGGCGGCGAGCGCGGCTTTTCCG
>@r2
NTTNTGATGCGGGCTTGTGGAGTTCAGCCGATCTGACTTATGTCATTACCTATGAAATGTGAGGACGCTATGCCTGTACCAAATCCTACAATGCCGGTGAAAGGTGCCGGGATCACCCTGTGGGTTTAT
>@r3
ATCGCCCGCAGACACCTTCACGCTGGACTGTTTCGGCTTTTACAGCGTCGCTTCATAATCCTTTTTCGCCGCCGCCATCAGCGTGTTGTAATCCGCCTGCAGGATTTTCCCGTCTTTCNGTGCCTTGNT
..........等等
直接看代码:
1 #encoding = utf-8 2 3 """ 4 简介:fasta文件中按id或者seqence长度排序 5 作者:刘自军 6 data:2017年5月17 21:38 7 """ 8 9 import sys 10 11 args = sys.argv 12 13 fasta = {} 14 with open(args[1]) as f: 15 16 for line in f: 17 line = line.strip() 18 if line.startswith('>'): 19 ID = line 20 fasta[ID] = '' 21 else: 22 fasta[ID] += line 23 24 if args[2] == 'id': 25 fasta = sorted(fasta.items(),key=lambda i:i[0]) #按id排序
#python3中废除类iteritems(),但用items()可以实现同样的效果 26 elif args[2] == 'len': 27 fasta = sorted(fasta.items(),key=lambda i:len(i[1])) #按每个序列的长度排序 28 else: 29 fasta = fasta.items() 30 31 for k,v in fasta: 32 print ('%s %s' %(k,v))