001、
root@PC1:/home/test# ls a.fastq test.py root@PC1:/home/test# cat a.fastq ## 测试fastq文件 @DJB775P1:248:D0MDGACXX:7:1202:12362:49613 TGCTTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCACACGTCTGAA + JJJJJIIJJJJJJHIHHHGHFFFFFFCEEEEEDBD?DDDDDDBDDDABDDCA @DJB775P1:248:D0MDGACXX:7:1202:12782:49716 CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAGATCGG + IIIIIIIIIIIIIIIHHHHHHFFFFFFEECCCCBCECCCCCCCCCCCCCCCC root@PC1:/home/test# cat test.py ## 测试程序 #!/usr/bin/python in_file = open("a.fastq", "r") dict1 = {} idx = 0 for i in in_file: idx += 1 i = i.strip() if idx % 4 == 1: key = i dict1[key] = "" elif idx % 4 == 2: dict1[key] = len(i) for i in dict1: print(i + "-------" + str(dict1[i])) in_file.close() root@PC1:/home/test# python test.py ## 执行程序 @DJB775P1:248:D0MDGACXX:7:1202:12362:49613-------52 @DJB775P1:248:D0MDGACXX:7:1202:12782:49716-------52
参考:https://www.jianshu.com/p/5ee54bea4cb0