000--functions for Tuple(the highlight ones) VS functions for List
001-- add one more tuple to original one with only 1 element, you need you use '' and ():
temp = ('one', 'two','four','five')
temp = temp[:2] + ('three',)+temp[2:]
002-- when you need to update the data (add,remove,change the value...), then use list; if you don't want any change for security issue, then use Tuple
003-- there is no Tuple comprehensions (it's enough with list comprehensions)